Research Highlight


The nPLEX chip loaded into the microarray spotting system. This technique allows for antibody conjugation on the nPLEX for exosome interaction testing. Source: MGH

Nano-plasmonic exosome (nPLEX) assays for exosome analysis and antibody validation

Exosomes are phospholipid nanovesicles secreted by mammalian cells that have recently been a topic of interest in cancer research and reports. These miniscule particles are released in large numbers by most types of cancers and often carry molecular information about the parent tumor cell: information that could be useful in diagnostic and tumor response to therapy situations. However, current methods of exosome analysis tend to be difficult to use in clinical settings where exosome concentration is low, or that require high throughput. A group of researchers at the Center for Systems Biology at Massachusetts General Hospital has recently developed a surface plasmon resonance (SPR) assay based on optical transmission through periodic nanoholes called the nano-plasmonic exosome (nPLEX) assay. This generation of the nPLEX sensor was designed for label-free detection of exosomes and validated across different ovarian cancer cell lines and ascites samples from ovarian cancer patients. A next generation nPLEX sensor is being created with the goal of establishing and validating the clinical utility of the sensor across a wide range of human cancers including glioblastoma multiforme, pancreatic, and ovarian cancer. One of the critical components in proteomic research, including exosome studies, is selecting the correct antibodies and validating their qualities in a high-throughput way. The affinity, specificity, reproducibility, and selectivity of antibodies tend to have some variation by many factors including vendor, clonality, target epitopes, species, and storage condition. Using microarray spotting technology, a protocol on high-throughput antibody validation will be established for nPLEX. Specifically, different antibodies (e.g. antibodies for CD63, a well known exosome surface marker) will be conjugated on the nPLEX chip using the microarray spotting system; antibodies will then be tested through exosome interactions. The antibody validation protocol is expected to reduce assay development costs and improve the accuracy of the nPLEX assays.

 

 


Trainee Research

CaNCURE provides trainees with a 6-month hands-on research experience and one-on-one mentoring by leading researchers in cancer nanomedicine.   Projects performed by current and past participants include:

Radiotherapeutic synergism of thermogelling cisplatin-loaded polymers for cervical cancer treatment

Investigating the use of iron chelator deferoxamine (DFO)-bearing PEG-like nanoprobes as a multifunctional agent for cancer therapy and PET imaging

Identifying genomic and compound dependencies in undifferentiated sarcomas

Protein-encapsulated nanoparticles for oral delivery of anti-mitotic agents in prostate cancer

Use of a Triblock Copolymer Hydrogel for Controlled Release of Cisplatin and BMN-673

Assessment of neoadjuvant therapy-induced atherosclerotic changes using ferumoxytol-enhanced MR imaging

Development of PSMA-targeting nanoparticles for positron emitting tomography imaging in prostate cancer using animal models

Assessing the reproducibility of MRI-based brain tumor measurements between both observers and MRI vendors

Quantitative Multimodal Imaging of Tumor Response to Radiation

Quantification of SPION accumulation in tumors using positive-contrast MRI

Digital diffraction diagnostics for lymphoma and HPV

T1-weighted imaging of primary pancreatic adenocarcinoma using magnetic ferumoxytol nanoparticles

Nano-plasmonic exosome (nPLEX) assays for exosome analysis and antibody validation

The Assessment and Comparison of Ferumoxtran as Contrast Imaging Agent in Patients with Pancreatic Cancers.

Biological mechanisms of gold nanoparticle-enhanced radiation therapy of prostate cancer

Combined delivery of targeted liposomal chemotherapeutics and photodynamic therapy to treat pancreatic cancer

Iron-chelating PEG-like nanoprobes as therapeutic and 89Zr/PET imaging agents

Combined Cisplatin and Olaparib nanoparticles for ovarian cancer therapy

Capture of circulating tumor DNA through the use of biotinylated poly-lysine affixed to gold nanoparticles

Development of a Point of Care Assay for Detecting High Risk HPV in Resource Limited Settings

Localized chemo- and chemo-radiation for the treatment of prostate cancer

Nanomedicine for Safe Healing of Bone Trauma

Uptake and localization of nanoparticles in prostate and lung cancer cells as a function of time and nanoparticle type

MCT1 Transporter Inhibition of IMR90 Cells Expressing Inducible Merkel Cell Carcinoma Small T Antigen

Co-delivery of protective substrate and chemotherapy drugs via lipid Bilayer Mesoporous Silica Nanoparticles


Soleil Doggett (Biology, '16) talks to her fellow peers about her research on oxygenating tumors to stimulate the anti-tumor immune response.

 


Trainee e-portfolios

Photo credit: Tom Kates Photography

While on co-op, trainees document their research in an e-portfolio.  This gives trainees the opportunity to provide regular updates on their research progress, reflect on training they are receiving, and explain how their research fits within the field of cancer nanomedicine.  These research e-portfolios can be accessed through individual trainee profiles.  The complete collection may be found here.

Check out this month’s featured e-portfolios by Rachel Fontana and Jordan Harris!

 


Presentation at CaNCURE Nanomedicine Day

At the completion of their co-op, trainees are provided with the opportunity to present their research to a wider audience.  In our 1st annual CaNCURE Nanomedicine Day, trainees prepared interactive, digital posters to display on electronic poster boards.  Over 100 faculty, students, and researchers attended our first event!

Check out the news article and congrats to all the poster winners!

Jordan Harris: Most Innovative Cancer Research Award
Jeremy Thong: Best Undergraduate Research Poster Award
Craig Pille: Most Promising Translational Research Award
Bryan Kynnap: Most Promising Basic Science Award
Jordan Harris: Top Chemical Engineering Poster Award