Metastasis to the brain from cancers such as breast, lung, and ovarian cancer presents a significant challenge in oncology, often leading to poor patient outcomes. Our research aims to tackle this problem by detecting extracellular vesicles (EVs) in cerebrospinal fluid (CSF) as biomarkers for brain metastasis. EVs are known to carry molecular signatures of their cells of origin, making them potential indicators of metastatic activity. In this project, we will isolate EVs from CSF samples of patients with primary cancers known to metastasize to the brain. We will analyze the EVs for specific protein and RNA markers associated with metastasis using advanced techniques such as nanoparticle tracking analysis (NTA) and next-generation sequencing (NGS). The goal is to identify distinct markers that can be used for early detection and monitoring of brain metastasis. This research could significantly improve the early diagnosis and treatment strategies for patients with metastatic cancer, ultimately enhancing survival rates and quality of life.
Northeastern University
